Application of reverse transcription loop-mediated isothermal amplification technology in the field of oncology
10.3760/cma.j.issn.1006-9801.2013.07.021
- VernacularTitle:反转录环介导等温扩增技术在肿瘤学领域的应用
- Author:
Yang HAN
- Publication Type:Journal Article
- Keywords:
Neoplasms;
Reverse transcription loop-mediated isothermal amplification;
Diagnosis
- From:
Cancer Research and Clinic
2013;25(7):493-495
- CountryChina
- Language:Chinese
-
Abstract:
Reverse transcription loop-mediated isothermal amplification(RT-LAMP)is a novel nucleic acid amplification technique which has been mainly used in a variety of viruses and other pathogens of gene detection.Compared with reverse transcriptase polymerase chain reaction (RT-PCR),the RT-LAMP has advantages of reaction simplicity,rapidly,cost-effective,detection sensitivity and specificity.Nowadays,RT-LAMP technology is gradually used in various medical fields,but it is still a new method especially in cancer diagnosis of tumor gene.The article reviews the application of the RT-LAMP technology in the cancer
