Effect of cyclosporine on the genetic expression of signal transducer and activator of transcription 1 in bladder cancer of rats and its clinical significance
10.3760/cma.j.issn.1006-9801.2013.08.005
- VernacularTitle:环孢素对大鼠膀胱肿瘤信号转导和转录活化因子1基因表达的影响及其临床意义
- Author:
Liang REN
;
Xiaopeng HU
;
Hang YIN
;
Wei WANG
;
Hang LIU
;
Xiaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Urinary bladder neoplasms;
Signal transducer and activator of transcription 1;
Gene expression;
Organ transplantation
- From:
Cancer Research and Clinic
2013;25(8):520-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cyclosporine on signal transducer and activator of transcription 1 (STAT1) genetic expression on bladder cancer in rats induced by BBN and its clinical significance.Methods Twenty SD rats were divide into experimental group or control group randomly.Ten samples of SD rats bladder cancer induced with BBN and cyclosporine simultaneously as experimental group,and 10 samples of SD rats bladder cancer induced with BBN only as control.Real time RT-PCR and immunohistochemistry stain were used to detect STAT1 mRNA and protein level expressions of bladder cancer in rats respectively.Results The STAT1 mRNA median expression fold was 4.5 (2.1-6.6) in experimental group and 5.6 (3.4-8.5) in control group.The STAT1 protein expression were 5 cases with (-),3 cases (+),2 cases (++) in experimental group and 0 case (-),5 cascs (+),5 cases (++) in control group.The expression of STAT1 mRNA and protein level of bladder cancer between experimental group and control group were both significant different (P ＜ 0.05).Conclusions Cyclosporine may stimulate the growth and development of bladder cancer through changing expression of some genes like STATI,and STAT1 maybe become one of the targets of chemoprevention for post-transplantation bladder cancer.
