An experimental study on the role of MSCs in inducing regulatory B cells and the underlying mechanisms

VernacularTitle:同种大鼠骨髓间充质干细胞诱导调节性B淋巴细胞的免疫负调节作用
Author: Yue WANG ; Jian NIU
Publication Type:Journal Article
Keywords: Rats; Mesenchymal stem cells; B-Lymphocytes; Immune tolerance
From: Chinese Journal of Organ Transplantation 2013;34(7):428-431
CountryChina
Language:Chinese
Abstract: Objective To observe the immunoregulatory roles of MSCs in inducing proliferation of B regulatory cells (Bregs).Method DA Rat MSCs were co-cultured with Wistar rat B cells.The levels of IL-10 and secrete proinflammatory cytokines in the supematants were determined by using ELISA.The proliferation and apoptosis of B cells after interaction with MSCs were analyzed by using FACS.In order to investigate whether Bregs are involved in the phagocytosis of immune complex (IC),B cells and Bregs were incubated with FITC-OVA-IC.The fluorescence intensity was detected by using immunofluorescence microscopy.Results MSCs induced B cells to differentiate into Bregs with high CD19,CD1d and CD5 expression and high IL-10 level.The proliferation of B cells was significantly suppressed after interaction with MSCs.Bregs had enhanced phagocytic capacity as compared with that of B cells.Bregs significantly inhibited maDC-induced CD4+ T cell proliferation compared to B cells,without depressing IL-2 or IFN-γ secretion.Conclusion MSCs induce freshly isolated B cells to differentiate into Bregs characterized by the ability to preferentially produce IL-10.