Association of non-HLA gene polymorphisms with CMV infection after hematopoietic stem cell transplantation
10.3760/cma.j.issn.0254-1785.2013.07.010
- VernacularTitle:非HLA基因单核苷酸多态性对造血干细胞移植后巨细胞病毒感染的影响
- Author:
Ziling ZHU
;
Xiaojin WU
;
Yufeng FENG
;
Lijun DAI
;
Huirong CHANG
;
Depei WU
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell transplantation;
Cytomegalovirus;
Polymorphism,single nucleotide
- From:
Chinese Journal of Organ Transplantation
2013;34(7):419-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the non-HLA gene polymorphisms that influence CMV infection after hematopoietic stem cell transplantation (HSCT).Method Non-HLA gene (ACE,CD14,MPO,MBL) single nucleotide polymorphisms were determined by using sequence-specific primer polymerase chain reaction (PCR-SSP) and sequencing in 64 pairs of donors and recipients before HSCT and the differences of non-HLA gene were analysed in CMV positive and negative patients Results The distribution of ACE gene single nucleotide polymorphism was DD (14/128,10.9％),ID (72/128,56.3％),and Ⅱ (42/128,38.8％).The distribution of CD14-159 allele gene single nucleotide polymorphism was CC (18/128,14.1％),CT (81/128,63.3％),and TT (29/128,22.7％).The distribution of MPO-463 allele gene single nucleotide polyrnorphism was G (100)/128,78.1％),A (2/128,1.6％),and GA (26/128,20.3％).The distribution of MBL gene single nucleotide polymorphism was H (28/128,21.9％),HL (73/128,57.0％),L (27/128,21.1％),Y (87/128,68.0％),YX (38/128,29.7％),X (3/128,2.3％),A (94/128,73.4％),AB (32/ 128,25.0％),and B (2/128,1.6％).The allele frequency of ACE,CD14 and MPO shoed no significant differcence between CMV positive and negative patients The gene frequency of MBL-HL was increased in CMV positive group.Conclusion MBL gene single nucleotide polymorphisrns may influence CMV infection after HSCT.
