miR-126 promotes endothelial progenitor cell migration and targets KANK2
10.3760/cma.j.issn.1007-631X.2013.08.015
- VernacularTitle:miR-126对内皮祖细胞增殖和迁移的影响以及靶基因的检测
- Author:
Qingyou MENG
;
Wenbin WANG
;
Zhixin CAI
;
Bin SHANG
;
Xiaoqiang LI
- Publication Type:Journal Article
- Keywords:
Micro RNAs;
Stem cells;
Cell proliferation;
Cell movement
- From:
Chinese Journal of General Surgery
2013;28(8):611-614
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of miR-126 (micro RNA-126) in rat endothelial progenitor cells (EPCs) proliferation and migration and the starget gene of miR-126 by bioinformatics and experimental survey.Method EPCs were transfected with control oligoes and miR-126 mimics or inhibitor by electroporation.MTT was performed to evaluate the growth of EPCs subjecting to miR-126 overexpression.Cell migration analysis was done by wound healing and transwell assay.The target genes of miR-126 were predicted by TargetScan and validated by Western blot.Result (1) miR-126 mimics promoted EPCs growth at 24 h post cell transfection (P ＜ 0.01).In contrast,the EPCs growth was immue from miR-126 application at 48 and 72 h.(2) Both the wound healing and transwell assay show that miR-126 promotes EPCs migration (P ＜ 0.01) and miR-126 inhibitor inhibits EPCs migration (P ＜ 0.01).(3)It is predicted that KANK2 is the potential target gene of miR-126 by TargetScan online software.(4) The results of Western blot indicated that miR-126 mimics repress the expression of KANK2 compared with NC but miR-126 inhibitor enhances KANK2 expression.Conclusions miR-126 has a transient effect on the promotion of EPCc growth.miR-126 promotes EPCs migration and targets KANK2 protein.
