Caveolin-1 inhibits vascular anastomotic stenosis and down regulates TNF-α expression
10.3760/cma.j.issn.1007-631X.2013.08.019
- VernacularTitle:Caveolin-1抑制血管吻合口狭窄及其对肿瘤坏死因子-α的作用
- Author:
Guoshan BI
;
Xianpeng DAI
;
Xin SHEN
;
Liming DENG
;
Jie JIANG
;
Hui LIU
;
Guozuo XIONG
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factors;
Surgical stomas;
Caveolin-1;
Stenosis
- From:
Chinese Journal of General Surgery
2013;28(8):624-627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of caveolin-1 on rabbit carotid artery anastomotic stenosis and its relationship with TNF-α.Methods 40 New Zealand white rabbits were randomly divided into normal group,surgical group,empty vector group and caveolin-1 transfecting group.Carotid artery end-to-end anastomosis was done in the rabbits except these in normal group.Five specimens were randomly taken on day 7 after surgery for Westem blot to detect the expression of caveolin-1 and TNF-α; The rest specimens were taken for HE staining at fourth week.The ratio of intima/media area were measured by Image-Pro Plus 6.0 software in order to observe the proliferation of intima.Results Compared with normal group,in surgical group intimal proliferation was significant,the intima/media ratio was significantly higher (P ＜ 0.05) ; Compared with surgical group,in caveolin-1 transfected group neointimal proliferation was not obvious,the intima/media ratio decreased (P ＜ 0.05).Western blot showed that:compared with the surgical group,caveolin-1 expression in transfected group was significantly higher (P ＜ 0.05) ; compared with normal group,the TNF-α expression in surgical group increased (t:41.28,P ＜ 0.05) ; Compared with surgical group,TNF-α expression in transfected group decreased significantly (t:36.37,P ＜ 0.05).Conclusions Caveolin-1 inhibits vascular anastomotic stenosis,possibly by down-regulating TNF-α expression.
