Role of complement 1q in hepatic ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2013.06.026
- VernacularTitle:补体C1q在大鼠肝脏缺血再灌注损伤中的作用
- Author:
Xiaobo FENG
;
Yan RAO
;
Jianjuan KE
;
Yanlin WANG
;
Zongze ZHANG
- Publication Type:Journal Article
- Keywords:
Reperfusion injury;
Liver transplantation;
Complement C1q
- From:
Chinese Journal of Anesthesiology
2013;33(6):746-748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of the complement 1 q (C1 q) in hepatic ischemia-reperfusion (I/R) injury in rats.Methods Sixty healthy male Sprague-Dawley rats,aged 3-4 months,weighing 180-200 g,were randomized into 2 groups:sham operation group (S group,n =12) and hepatic I/R group (I/R group,n =48).Hepatic specimens were obtained at 1,3,6 and 24 h of reperfusion and were then cut and stained with haematoxylin and eosin for examination of histological changes of the liver (with light microscope) and for determination of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content (by colorimetric method),expression of Clq mRNA (using real-time PCR) and expression of Clq (by using Western blot).Results Compared with S group,the activity of SOD was gradually decreased,the content of MDA was gradually increased,and the expression of Clq and Clq mRNA was gradually up-regulated and peaked at 3 h of reperfusion with the prolongation of reperfusion time in I/R group (P ＜ 0.05).The pathological changes of the liver were aggravated with the prolongation of reperfusion time in I/R group.Conclusion Activation of C1 q is involved in hepatic I/R injury in rats.
