Changes in expression of annexin A2 in lung tissues in rats with hepato-pulmonary syndrome

VernacularTitle:肝肺综合征大鼠肺组织膜联蛋白A2表达的变化
Author: Yang CHEN ; Bin YI ; Bing CHEN ; Yong YANG ; Baoli ZU ; Kaizhi LU
Publication Type:Journal Article
Keywords: Hepatopulmonary syndrome; Annexin A2; Lung
From: Chinese Journal of Anesthesiology 2013;33(5):599-601
CountryChina
Language:Chinese
Abstract: Objective To evaluate the changes in the expression of annexin A2 (ANXA2) in lung tissues in rats with hepato-pulmonary syndrome.Methods Healthy 3-4-month-old Sprague-Dawley rats of both sexes,weighing 220-250 g,were randomly divided into 3 groups:control group (group C,n =10),sham operation group (group S,n =10) and hepatopulmonary syndrome group (group HPS,n =20).The rats were anesthetized with intraperitoneal 3％ pentobarbital sodium 0.1 ml/100 g.Hepatopulmonary syndrome was produced by chronic ligation of the common bile duct.After 5 weeks,the rats were sacrificed and lungs were removed for determination of ANXA2 and smooth muscle actin α (SM-α-actin) mRNA and protein expression in lung tissues by RT-PCR and Western blot,respectively.Results There was no significant difference in the expression of ANXA2 and SM-α-actin mRNA and protein between groups C and S (P ＞ 0.05).The expression of ANXA2 and SM-α-actin mRNA and protein was significantly higher in group HPS than in groups C and S (P ＜ 0.05).Conclusion The expression of ANXA2 in lung tissues is up-regulated in rats with hepato-pulmonary syndrome.