Effect of recombinant human endostatin on the radiosensitivity of esophageal carcinoma cells and underlying mechanism
10.3760/cma.j.issn.0254-5098.2013.04.003
- VernacularTitle:重组人血管内皮抑素对食管癌细胞的放射增敏作用及其机制
- Author:
Zhiqiang SUN
;
Jingping YU
;
Zhiming ZHANG
;
Jian WANG
;
Dongqing LI
;
Qinghong MENG
;
Suping SUN
- Publication Type:Journal Article
- Keywords:
Recombinant human endostatin;
Esophageal carcinoma cells;
Radiosensitivity
- From:
Chinese Journal of Radiological Medicine and Protection
2013;33(4):346-350
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of recombinant human endostatin (rhES)on the radiosensitivity of esophageal squamous cells KYSE-150 and its preliminary mechanism.Methods Cells were divided into four groups:control group without treatment,rhES group treated with recombinant human endostatin,radiation alone group exposed with X-rays,and combination group exposed with X-rays plus endostatin.Colony formation assay was used to measure cell survival fraction.A single-hit multi-target model was used to fit cell survival curve and calculate the sensitive enhancement ratio (SER).Influence of rhES combined with X-ray radiation on cell cycle and apoptosis was measured by flow cytometry.Expressions of Cyclin B1,Cyclin D1,Bcl-2 and Bax mRNAs were determined by RT-PCR.Protein expressions of HIF-1α,VEGF,and VEGFR were determined by Western blot.Results D0,Dq and SF2 value of KYSE-150 cells decreased along with the concentration of rhES.At D0dose,the SER for 100 and 200 μg/ml rhES was 1.14 and 1.27,respectively.Compared with the radiation alone group,the apoptosis rate and bax expression increased,while the expressions of VEGF and HIF-1α decreased in the combination group (t =7.97,3.02,117.55,7.22,P ＜ 0.05).Conclusions rhES has radiosensitive effect on esophageal carcinoma cells KYSE-150 in vitro by inhibiting the expressions of HIF-1α and VEGF,regulating bax expression,and inducing apoptosis.
