Protective effect of 1,25-(OH)2D3 on radiation-induced bone marrow microenvironment injury
10.3760/cma.j.issn.0254-5098.2013.04.001
- VernacularTitle:1,25-(OH)2D3对辐射损伤小鼠骨髓微环境的保护作用
- Author:
Xiaoyu CHEN
;
Aizhen ZHU
;
Chengcheng LIU
;
Jincan ZHU
;
Gexiu LIU
- Publication Type:Journal Article
- Keywords:
Bone marrow microenvironment;
Radiation;
Adipogenesis;
1,25-(OH)2D3
- From:
Chinese Journal of Radiological Medicine and Protection
2013;33(4):337-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of 1,25-(OH) 2D3 on radiation-induced bone marrow microenvironment injury and to explore the related molecular mechanism.Methods Sixty 7-week old male BALB/c mice were randomly divided into control group without any treatment; radiation group exposed to 6.0 Gy 60Co γ-rays with DMSO,and 1,25-(OH)2 D3 + radiation group treated with 1,25-(OH)2D32.5 μg/kg dissolved in DMSO each day and 6 Gy of γ-rays.The body weight and peripheral white blood cells,femur bone marrow histology,and the proportion of adipocyte area were measured.The expression of peroxisome proliferator-activated receptor-gamma (PPARγ) was detected immunohistochemistrically at 8 d after irradiation.Results After irradiation,the number of white blood cells and the body weight decreased obviously,and the percentage of adipocyte area was increased significantly.Compared with radiation group,1,25-(OH)2D3 reduced the decrease rate of body weight (t =-2.23,-2.34,P ＜ 0.05),partly recovered the number of white blood cells at 4 or 8 d after irradiation(t =-4.99,-4.46,P ＜ 0.05),and reduced the proportion of adipocyte area (t =-3.75,-2.10,P ＜ 0.05).With immunohistochemistrical assay,it was found that 1,25-(OH) 2D3 inhibited adipogenesis by reducing the expression of PPARγ.Conclusions 1,25-(OH) 2 D3 decreases radiationinduced adipogenesis and hence protects the bone marrow microenvironment from radiation damage.
