Mechanism of curcumin-enhanced radiosensitivity in pancreatic cancer cells PANC-1

VernacularTitle:姜黄素提高人胰腺癌PANC-1细胞放射敏感性的机制研究
Author: Minghua BAI ; Yang ZHANG ; Baofeng WANG ; Hongbing MA
Publication Type:Journal Article
Keywords: Curcumin; γ-rays; PANC-1 cells; P21; Pancreatic cancer
From: Chinese Journal of Radiological Medicine and Protection 2013;33(4):360-363
CountryChina
Language:Chinese
Abstract: Objective To investigate the combination effect of curcumin and γ-ray irradiation on PANC-1 cells in vitro.Methods PANC-1 cells were exposed to γ-rays in the presence or absence of curcumin.MTT assay was performed to evaluate cell viability.The expression of P21 was evaluated with RT-PCR and Western blot.Cell cycle distribution and apoptosis were tested by flow cytometry.Results Compared with the γ-ray irradiation group,combination treatment of curcumin and irradiation decreased the cell viability (t =6.72,P ＜ 0.01) and increased the percentage of cells in S-phase (t =4.78,P ＜ 0.05),apoptosis rate (t =6.58,P ＜ 0.01),P21 protein and mRNA expression (t =5.72,5.63,P ＜ 0.01) in PANC-1 cells.Conclusions Curcumin increases the radiosensitivity of PANC-1 cells,which may have clinical implication on radiotherapy of pancreatic cancer.