Role of tumor necrosis factor like ligand-1A aberrance in the generation and differentiation of peripheral blood Th17 in rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2013.08.004
- VernacularTitle:肿瘤坏死因子样配体1A对类风湿关节炎患者外周血Th17产生及分化的调节作用
- Author:
Min ZHOU
;
Rui LIU
;
Xia LI
;
Lingyun SUN
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Tumor necrosis factor like ligand-1A;
Th17;
Death receptor 3
- From:
Chinese Journal of Rheumatology
2013;17(8):518-521
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of TL1A in the generation and differentiation of peripheral blood Th17 in rheumatoid arthritis.Methods The peripheral blood mononuclear cells (PBMCs) of RA were isolated and stimulated with PHA in the presence or absence of TL1A.Naive CD4+ T cells from RA was cultured under Th17-polarizing conditions with or without TL1A.The percentage of Th17 was detected by flow cytometry (FCM) analysis.The DR3 expression on CD4+ T cells from PBMCs of RA patients and healthy controls (HC) were analyzed by using FCM.Data were analyzed with t test and U test.Results TL1A could significantly up-regulate the percentage of Th17 in PBMCs of RA [(7.5±2.3)％ vs (5.2±1.5)％,t=2.647,P＜0.05].Compared to the HC groups,TL1A could significantly induce Th17 differentiation from naive T cells [(37.7±1.9)％ vs (29.5±2.0)％,t=6.455,P＜0.05].The percentage of CD4+DR3+ T cell in PBMCs of RA [(0.56±0.87)％] was significantly higher than that of HC [(0.13±0.04)％,P＜0.05].The percentage of CD4+DR3+ T cell in PBMCs when stimulated with PHA was increased in RA patients,[(4.51±1.34)％ vs (1.11±0.29)％,t=2.915,P＜0.05],but no obvious increase in HC [(0.199±0.104)％ vs 0.072％±0.029)％,t=1.644,P=0.1988].Conclusion TL1A can promote the generation and differentiation of Th17 in the PBMCs of RA,and this effect may be mediated by the binding of TL1A with DR3.
