Study on Foxp3 promoter region methylation of mononuclear cells in patients with rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2013.04.011
- VernacularTitle:类风湿关节炎患者单个核细胞Foxp3启动子区甲基化研究
- Author:
Yiqun HAO
;
Xiumei LIU
;
Xin YAN
;
Jie YANG
;
Zili FU
;
Dongping LUO
;
Kai WANG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
T-lymphocytes,helper-inducer;
Peripheral blood mononuclear cells;
Foxp3
- From:
Chinese Journal of Rheumatology
2013;17(4):264-267
- CountryChina
- Language:Chinese
-
Abstract:
Objective By detecting the expression levels of Foxp3 in CD4+CD25+ regulatory T cells of peripheral blood mononuclear cells (PBMCs) in rheumatoid arthritis (RA),and the Foxp3 gene promoter region methylation to explore its role in the pathogenesis of RA.Methods Twenty-five RA patients and 10 healthy controls were selected,and the PBMCs were extracted by density gradient centrifugation.Foxp3 expression levels of CD4+CD25+ regulatory T cells were detected by flow cytometry.The real-time fluorescence quantitative PCR assay was used to detect the Foxp3 mRNA expression in PBMCs; and bisulfate processing gene sequencing was used to determinethe differences in Foxp3 gene promoter sequence methylation level of PBMCs.The comparison between groups was analyzed using one-way ANOVA; two sets of qualitative data were compared using Fisher's exact test.Results The expression levels of Foxp3 mRNA in the CD4+CD25+regulatory T cells of active RA patients (2.31±0.25) was significantly lower than inactive RA group (3.68±0.26) and healthy controls (5.67±0.34),the difference was statistically significant (P＜0.05).The Foxp3 mRNA expression level in inactive RA group was lower than that of the healthy controls (P＜0.05).Foxp3 promoter region-67,-74 sites of methylation level in PBMCs of RA patients (46％) was significantly higher than that of the healthy controls (6％).Conclusion Reduction in the number of CD4+CD25+ regulatory T cells may be involved in the pathogenesis of RA and Foxp3 gene promoter methylation levels plays a key role in this process.
