The expression of interleukin-27 in peripheral blood of patients with ankylosing spondylitis and the mechanism of its action on CD4+ T cells

VernacularTitle:白细胞介素-27在强直性脊柱炎患者外周血的表达及其对CD4+T细胞作用机制研究
Author: Jianhua SHENG ; Jian WU ; Yanping GONG ; Yanhong SHEN ; Tingwang JIANG
Publication Type:Journal Article
Keywords: Spondylitis,ankylosing; Interleukin-27; CD4 lymphocyte count
From: Chinese Journal of Primary Medicine and Pharmacy 2013;20(20):3044-3046
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of interleukin-27 (IL-27) in proliferation and differentiation of CD4+ T cells in ankylosing spondylitis(AS).Methods CD4+ T cells were separated from peripheral blood which collected from AS patients and health controls(HCs).Cells proliferation was detected by CCK-8 kit,and cytokines were analyzed by ELISA.Real-time PCR was used to determine the mRNA expression of T-bet and GATA3.Results Serum IL-27 level in patients with AS was obviously higher than that in HCs(P ＜0.01).The proliferation rate of CD4+ T cells and the level of IFN-γin cultured medium in AS were higher than those in HCs group after IL-27 stimulation (P ＜ 0.01).IL-27 could induce T-bet mRNA expression in CD4+ T cells in AS (t =14.3,P ＜ 0.01),but no change was found in GATA3 mRNA expression.Conclusions IL-27 could induce the proliferation of CD4+ T cells and the activation of T-bet pathway in AS.Furthermore,Th1 immune response and related cytokines could be induced by IL-27 in AS.These implicate that IL-27 may play an important role in AS related inflammation.