Detection and analysis of MP-DNA in BALF of mycoplasma pneumoniae pneumonia in children

VernacularTitle:肺泡灌洗液肺炎支原体DNA检测对儿童支原体肺炎的诊断价值
Author: Chenyu ZHANG ; Bangning CHENG ; Jiayan HAO ; Xiaoji SU
Publication Type:Journal Article
Keywords: Mycoplasma pneumoniae pneumonia; Bronchoalveolar lavage fluid
From: Chinese Journal of Primary Medicine and Pharmacy 2013;20(19):2945-2946
CountryChina
Language:Chinese
Abstract: Objective To study the value of MP-DNA in the early diagnosis of pneumonia by analyzing the result of MP-DNA in bronchoalveolar lavage fluid(BALF) and MP-IgM antibodies in serum.Methods 103 hospitalized children were detected for MP-DNA in BALF(positive:DNA copies ＞ 102/ml) and MP-IgM antibodies in serum.Results The MP-DNA positive in BALF and the MP-IgM positive in serum in children with pneumoina were significantly higher than those in children with foreign body in bronchus (x2 =13.00,4.11,all P ＜ 0.05).33.01％ of 103 children was MP-DNA positive,19.43％ of 103 children was MP-IgM positive.The MP-DNA positive in BALF was significantly higher than the MP-IgM positive in serum(x2 =4.92,P ＜0.05).Conclusion The samples of MP-DNA in BALF were collected more difficulty than the samples of MP-IgM in serum,but the detection of MP-DNA can avoid the false negative,which may be of value in the diagnosis and therapy of MPP.