Application of high resolution melting analysis in detecting IDH1 mutation in glioma patients
10.3760/cma.j.issn.1008-6706.2013.22.009
- VernacularTitle:高分辨率熔解曲线分析法检测胶质瘤中异柠檬酸脱氢酶1基因突变
- Author:
Haiyan ZHANG
;
Jie WANG
;
Xiaodong CHEN
;
Wei ZHANG
;
Jiang XIAN
- Publication Type:Journal Article
- Keywords:
Glioma;
Mutation;
IDH1 gene;
High resolution melting curve analysis
- From:
Chinese Journal of Primary Medicine and Pharmacy
2013;20(22):3379-3380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility of detecting the mutation of IDH1 in glioma patients by high resolution melting(HRM) curve analysis.Methods The gene mutation of IDH1 was detected by HRM method in 9 surgical resection paraffin specimens of glioma,and the result was verified by gene sequencing.Results 6 cases of R132H(CGT > CAT) mutation and 1 case of R132C(CGT > TGT) mutation were found by HRM method.The resluts of direct gene sequencing were consistent with HRM method.Conclusion The HRM method in detecting IDH1 mutation is more efficient and convenient than direct sequencing.Moreover,it has advantages of low-cost and suitable for clinical test.