Effects of L.Lactis recombinant heme oxygenase-1 gene on the intestinal barrier and blood-gas analysis in rats with hemorrhagic shock
10.3760/cma.j.issn.1008-6706.2013.19.005
- VernacularTitle:血红素加氧酶-1重组乳酸乳球菌对失血性休克大鼠肠黏膜屏障和血气分析的影响
- Author:
Xinyue GAO
;
Ziliang QIAN
;
Congcai REN
- Publication Type:Journal Article
- Keywords:
Heat-shock proteins;
Shock,hemorrhagic;
Intestinal mucosa;
Blood gas analysis;
Muridae
- From:
Chinese Journal of Primary Medicine and Pharmacy
2013;20(19):2890-2892
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protection mechanism of HO-1 with the gas-blood analysis and MAP of femoral artery in rats with hemorrhagic shock.Methods A model of hemorrhagic shock was established in 20 SD healthy clean male rats.The rats were randomly divided into the L.Lactis recombinant HO-1 gene group (HO group)and phosphate buffer solution group (PBS group).The gas-blood analysis and MAP of femoral artery in hemorrhagic shock were compared during the test.The mortality rate,MPO activity,bacterial translocation,the pathologic and content of HO-1,TNF-α and IL-10 in the low intestine were detected and compared 1h after resuscitation.Results Compared to PBS group,the mortality rate,Chiu's grade,bacterial translocation and MPO activity in HO group were significantly decreased[10％ vs40％,(1.51 ±0.23) points vs(2.15 ±0.48) points,44.4％ vs 100.0％,(0.16 ±0.05)U/g vs (0.99 ± 0.28)U/g,all P ＜0.05],PaO2 and MAP during the resuscitation,the content of HO-1 and the gray level of IL-10 were significantly increased.Conclusion L Lactis recombinant HO-1 gene has the virtue to maintain the higher level of PaO2 and MAP,which is beneficial to the intestine mucosa barrier and anti-inflammation response of low intestine significantly.
