Expressions and correlation of Glil and β-catenin in the colorectal carcinoma
10.3760/cma.j.issn.1006-9801.2013.03.009
- VernacularTitle:大肠癌中Gli1和β-catenin的表达及其相关性研究
- Author:
Hua REN
;
Jianping ZHAO
;
Yirui ZHAO
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Glil;
β-catenin
- From:
Cancer Research and Clinic
2013;(3):175-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Glil and β-catenin,which are central molecules of Hedgehog and Wnt signaling pathway,in colon cancer tissues and cell lines,and evaluate their relationship.Methods RT-PCR and Western blot were used to determine the expression of Glil and β-catenin in colon cancer tissues and cells.Interaction between endogenous Glil and endogenous 3-catenin was examined using immunoprecipitation method.Results Compared to corresponding normal tissues,the expression of Gli1 and β-catenin raised raising with one accord,the average growth rates were 42.69,72.11 respectively (P ＜ 0.05).In colon cancer cell lines,the expression levels of Glil and β-catenin increased,which were 52.54,17.23,5.54 and 5.30,6.34,2.78 (all P ＜ 0.05).Both of them were enhanced in three Wnt aberrant active cells visibly.The immunoprecipitation result indicated that there was an interaction between Glil and β-catenin in colon cancer cells.Conclusion The expression of Glil and β-catenin rise congruously in both colon cancer cells and tissues,and there is an interaction between them.This interaction between two key components of Hedgehog and Wnt signaling may suggest the possible crosstalk manner in these pathways.
