Anti-tumor effects of supernatant from B16 melanoma cells mixedly cultured with lymphocytes and macrophages in vitro
10.3760/cma.j.issn.1006-9801.2013.03.003
- VernacularTitle:B16黑色素瘤细胞、淋巴细胞和巨噬细胞混合培养上清的体外抗肿瘤作用
- Author:
Xiangyu ZHANG
;
Panpan FENG
;
Yiyang WEN
;
Yingsong QIN
- Publication Type:Journal Article
- Keywords:
Chemotaxis;
Neoplasms;
Tumor infiltrating T lymphocytes;
Cell-killing effect;
Tumor culture supernatant
- From:
Cancer Research and Clinic
2013;(3):152-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-tumor effects of mixed cultured B16 melanoma cells supernatant.Methods The supernatant from purely cultured B16 melanoma cells or mixedly cultured B16 melanoma cells with lymphocytes and macrophages at indicated time points were collected,respectively.The chemotaxis of the two different cell supernatants was determined by Boyden room method.The activation effects towards lymphocytes of the two different supernatants were determined by CCK-8 method.Results When the cells were mixed cultured for 0.5,1,2,4,8 and 12 h,the numbers of lymphocytes to travel from the upper well to the bottom well were (1.00±0.82) × 104,(7.00±1.63) × 104,(9.50±0.58) × 104,(11.25±2.36) ×104,(17.25±1.71) × 104 and (21.50±1.29) × 104,respectively.When the cells were purely cultured for 0.5,1,2,4,8 and 12 h,the numbers of lymphocytes to travel from the upper well to the bottom well were (0.00±0.00) ×104,(0.25±0.50) × 104,(1.75±0.96) × 104,(5.25±0.96) × 104,(5.75±1.26) × 104 and (10.75±3.20) × 104,respectively.The mixed cultured group showed higher chemotaxis effects towards lymphocytes in comparison with the purely cuhured one at the same points except for 0.5 h (P ＜ 0.05).The mixed cultured group showed higher activation effects towards lymphocytes in comparison with the purely cultured at the same points except for 0.5 and 1 h (P ＜ 0.05).Each group showed higher chemotaxis and activation effects towards lymphocytes when they were cultured for 12 h than the other time points (P ＜0.05).Conclusion The supernatant from mixed cultured cells shows much higher chemotaxis and activation effects towards lymphocytes to kill tumor cells.
