Effect of cyclosporine on the genetic expression of matrix metalioproteinase-3 of bladder cancer in rats and its clinical significance
10.3760/cma.j.issn.1006-9801.2013.06.012
- VernacularTitle:环孢素对大鼠膀胱肿瘤基质金属蛋白酶3基因表达的影响及其临床意义
- Author:
Liang REN
;
Xiaopeng HU
;
Hang YIN
;
Wei WANG
;
Hang LIU
;
Xiaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Urinary bladder neoplasms;
Cyclosporine;
Matrix metalloproteinase 3;
Gene expression;
Organ transplantation
- From:
Cancer Research and Clinic
2013;(6):403-405
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cyclosporine on matrix metalloproteinase-3 (MMP-3)’ s genetic expression on bladder cancer in rats induced with BBN and its clinical significance.Methods Twenty SD rats were divide into experimental group or control group randomly.Ten samples of SD rats bladder cancer induced with BBN and cyclosporine simultaneously and 10 samples of SD rats bladder cancer induced with BBN only as control were used to observe the effect of cyclosporine on MMP-3’ s genetic expression.Real time RT-PCR and immunohistochemistry stain were used to analyze MMP-3 mRNA and protein levels of bladder cancer in rats respectively.Results The MMP-3 mRNA median expression were 7.6 (4.2-9.1) in experimental group and 4.7 (2.8-7.7) in control group.The MMP-3 protein expression were 1 case with (-),4 cases (+),5 cases (++) in experimental group and 3 cases (-),4 cases (+),3 cases (++) in control group.The differences of MMP-3 mRNA and protein levels of bladder cancer between experimental group and control group were both significant (P ＜ 0.05).Conclusion Cyclosporine may stimulate the growth and development of bladder cancer through changing expression of some genes like MMP-3,and MMP-3 maybe become one of the targets of chemoprevention for post-transplantation bladder cancer.
