Effect of antifreeze or antioxidant at different cryopreservation conditions on activity of adipose cells
10.3760/cma.j.issn.1671-0290.2013.03.017
- VernacularTitle:防冻剂或抗氧化剂在不同低温保存条件下对脂肪细胞活性的影响
- Author:
Shaohua WANG
;
Aijuan CHENG
- Publication Type:Journal Article
- Keywords:
Fat granules;
Cryopreservation;
Antifreeze;
Glucose transfer;
Dimethyl sulfoxide (DMSO);
Trehalose
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2013;(3):215-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of different antifreeze or antioxidant at different cryopreservation conditions on the activity and the morphology of adipose cells.Methods After purification,fat granules were divided into 5 groups; the same volume of antifreeze or antioxidants were added in each group,stored at-20℃ (refrigerator),-80℃ (ultra-low-temperature refrigerator) and-196 ℃ (liquid nitrogen) ; the amount of glucose transfer was determined and the morphological changes were observed for each group after two weeks,two months,and three months.Results Fat activity of the group adding both dimethyl sulfoxide (DMSO) and trehalose was significantly higher than that in other groups at the same temperature and the same time point.The decreased amount of glucose transfer of 80℃ group and 196℃ group was significantly lower than that of 20℃ group,and that in groups of each 196℃ were greater than that in groups of each 80℃.Conclusions Antifreeze can obviously keep activity of fat cells.Preservative effect of DMSO is better than trehalose,and that of both combination is much better.Liquid nitrogen storage of adipose tissue for three months and fat granules stored at 80℃ for two months are still suitable for clinical use.
