Effect of platelet-rich fibrin on proliferation and adipogenic differentiation of adipose-derived stem cells

VernacularTitle:富血小板纤维蛋白对人脂肪干细胞增殖和成脂分化的影响
Author: Dong CUI ; Teng ZHANG ; Jiansheng DIAO ; Chenggang YI ; Shuzhong GUO
Publication Type:Journal Article
Keywords: Adipose-derived stem cells (ADSCs); Platelet-rich fibrin (PRF); Cell proliferation; Adipogenic differentiation
From: Chinese Journal of Medical Aesthetics and Cosmetology 2013;(3):203-206
CountryChina
Language:Chinese
Abstract: Objective To study the effect of autogeneic platelet-rich fibrin (PRF) on proliferation and adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro.Methods ADSCs were isolated from adipose tissue obtained from donors undergoing liposuction and were cultured,and underwent identification.ADSCs at passage 3 were divided into three groups:test groups were cultured with 1PRFM and 2PRFM,and control group was cultured without PRF membrane.Then the growth of the cells was observed by inverted microscope.MTT method was used to observe cell proliferation activity at days 1,2,3,4,5,6 and 7 after culture.Adipogenic differentiation of ADSCs was observed and quantified by oil red O staining at days 3,5,7,9,11 and 14.Results Cell proliferation and adipogenic differentiation would be increased with the PRFM,There were significant differences among three groups.Conclusions PRF could significantly promote proliferation and adipogenic differentiation of ADSCs.