Sequencing of Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium and application for PCR to differentiate them
- Author:
Nguyen Trong Tue
- Publication Type:Journal Article
- Keywords:
Flic gene;
Salmonella typhi;
Salmonella paratyphi A;
Salmonella paratyphi B;
Salmonella paratyphi C;
Salmonella typhimurium;
PRC
- MeSH:
Polymerase Chain Reaction;
Salmonella
- From:Journal of Preventive Medicine
2005;15(5):36-41
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
All Flic genes of Salmonella typhi, S. paratyphi A, S. paratyphi B, S. paratyphi C, and S. typhimurium code for phase 1 of H antigen (H:1) (d, a, b, c and i antigen respectively). The genes were sequenced on Sanger's principle by automatic sequenser (ABI, 3100 Avant, Genetic Analyser). The primer pairs for the Salmonella species were designed on the basis of the collected sequences. The results showed that PCR with these primers can clearly differentiate the five Salmonella strains, especially between S. paratyphi B and S. typhimurium.
