Effects of CTGF gene silencing on expression of type Ⅰ collagen in a nude mouse model
10.3760/cma.j.issn.1671-0290.2013.02.014
- VernacularTitle:结缔组织生长因子基因沉默对人体瘢痕疙瘩移植动物模型中Ⅰ型胶原表达的影响
- Author:
Yong DU
;
Peisheng JIN
;
Aijun ZHANG
;
Changbo TAO
;
Xueyang LI
;
Qiang LI
;
Caiqi SHEN
- Publication Type:Journal Article
- Keywords:
Keloid;
RNA interfering;
Connective tissue growth factor;
Inside intervention;
Animal Model
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2013;(2):126-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the specific silencing of connective tissue growth factor (CTGF) in a nude mouse keloid model,using RNA interference (RNAi) technique,and to provide the basis for gene therapy of keloid.Methods The nude mouse keloid model was established,and then transfected in vivo with well-amplifiating plasmid.The mRNA expression levels of CTGF mRNA and type Ⅰ collagen mRNA were detected with reverse transcription-polymerase chain reaction (RT-PCR).The distribution and protein expression levels of CTGF and type Ⅰ collagen were determined quantitatively using immunohistochemistry.Results The expression of CTGF at mRNA and protein levels was decreased in the experiment group,and the expression of type Ⅰ collagen at mRNA and protein levels was also decreased after transfection,as compared with negative control group and blank group,with significant difference between groups (P＜0.05).Moreover,the expression of type Ⅰ collagen and CTGF was positively correlated (r=0.979).Conclusions Keloid type Ⅰ collagen can be decreased through in vivo inhibiting CTGF expression.The transfection of CTGF gene in vivo may have effects on type Ⅰ collagen generation,and thus inhibit the keloid growth.
