Constructive tissue engineering bone with BMSC sheets and PLGA scaffold

VernacularTitle:骨髓基质干细胞片层复合聚乳酸羟基乙酸支架构建组织工程骨
Author: Chao YAO ; Ningyi LI ; Fengzhi LIU ; Lingling WANG ; Yueyuan YU ; Aihua SUI
Publication Type:Journal Article
Keywords: Bone marrow stromal stem cell; Cell sheet; Temperature responsive culture dish; Polylactic-co-glycolic acid; Tissue-engineering bone
From: Chinese Journal of Medical Aesthetics and Cosmetology 2013;(2):118-121
CountryChina
Language:Chinese
Abstract: Objective To construct bone marrow stem cell sheets and to investigate its effects in the process of osteogenesis.Methods BMSCs were differentiated into osteoblasts and then seeded into a temperature responsive culture dish to construct BMSC sheets.PLGA scaffolds in which both BMSC suspension and BMSC sheets were added,were implanted into the left side of the dogs' mandible.In the other side,PLGA scaffolds that were not wapped with BMSC sheets were implanted as control.At 16 weeks,the samples were processed for radiological analysis and histological examination.Results Cells in the BMSC sheets grew well.In the experimental side,the optical density of the samples was higher than that of the control side (P＜0.05) and plenty of lamellar bones and Haversian system were observed.Conclusions The formation of lamellar bones can be promoted by PLGA scaffolds and BMSC sheets in the process of tissue engineering bone reconstrution.