Effect of recombinant human basic fibroblast growth factor with different concentrations upon inducing hASCs to differentiate into adipose cells
10.3760/cma.j.issn.1671-0290.2013.02.016
- VernacularTitle:重组人碱性成纤维细胞生长因子对人脂肪干细胞分化为脂肪细胞的影响
- Author:
Yangyang XU
;
Nan JIANG
;
Liu YANG
;
Yong ZHAO
;
Weiwei GU
;
Jing CAO
;
Menglin ZHU
- Publication Type:Journal Article
- Keywords:
Human adipose-derived stem cells (hASCs);
Recombinant human FGF-basic;
Adipocyte;
Differentiate
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2013;(2):134-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of the exogenous recombinant human FGF-basic with different concentrations upon inducing human adipose-derived stem cells (hASCs) to differentiate into adipose cells,and the optimum concentration of exogenous rh-bFGF by experimental research.Methods hASCs were isolated and extracted by enzymatic digestion from the liposuction aspirate.hASCs using adipogenic supplement were divided into experimental group and blank group:the experimental group of adipogenic supplement was divided into adding the exogenous rh-bFGF 10 ng/ml,20 ng/ml and 40 ng/ml,the blank group of adipogenic supplement was cultured without exogenous rh-bFGF.MTT method was used to detect the adipocytes proliferation.The oil red O staining was used in the qualitative analysis on the time of newly forming adipocyte cells.Western blot was used to detect the effects of rh-bFGF on the expression of lipid droplets surface protein CIDEC at different stages during the culture.Results The experimental group could obviously shorten the period of inducing hASCs to differentiate into adioicytes,and promote the proliferation of adipocytes.The formation rate and the proliferation of adipocytes in the group adding 40 ng/ml rh-bFGF were superior to those in the experimental group else and blank group.The average time of the newly formed lipid droplets by adding 40 ng/ml rh-bFGFwas (11.5±1.9)h.The average absorbance of cell proliferation by adding 40ml rh-bFGF was 0.52 ±0.10.The CIDEC expression quantity of adding 40 ng/ml rh-bFGF group was also superior to that in the experimental group and blank group.Conclusions rh-bFGF in hASCs adipogenic supplement could promote the proliferation of adipocytes and dramatically accelerates the program of hASCs differentiating to adipocytes,in which the optimum concentration of rh-bFGF is 40ng/ml.
