Comparison of proliferation and differentiation capacity of tissue-engineered skin built by different passages of keratinocytes
10.3760/cma.j.issn.1671-0290.2013.02.013
- VernacularTitle:不同代次角质形成细胞构建组织工程皮肤的增殖分化能力比较
- Author:
Qiaoyu XU
;
Guihong YANG
;
Tao YANG
;
Duan WANG
;
Yuangang LU
;
Jinjin WU
- Publication Type:Journal Article
- Keywords:
Keratinocyte;
Tissue-engineered skin;
CK1/CK10;
CK5/CK14;
Ki-67
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2013;(2):122-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the epidermal shape built by different passages of keratinocytes and its ability of proliferation and differentiation in three-dimensional conditions.Methods Different passages of keratinocytes were used to construct tissue-engineered skin.The morphology of the tissue-engineered skin was observed with HE and PAS staining,while CK1/CK10,CK5/CK14,Ki67 were detected by immunohistochemical assays.Results All the tissue-engineered skin had a significant dermoepidermal structure.The stratification of 1st and 2nd passage skins were better,and 2nd passage epidermis was thicker than that in other passages (P＜0.05).Dermoepidermal structure in collagen type Ⅳ group binded more tightly,but collagen type Ⅳ had little effect on the thickness of the epidermis (P＞0.05).In collagen type Ⅳ group PAS stain was negative,indicating type Ⅳ collagen was unable to promote the reconstruction of BM in vitro.The Ki-67 proliferation index of the 2nd keratinocyte was similar to the normal skin,the remaining passages keratinocyte proliferation gradually decreased (P＜0.05) ; the 1st and 2nd passage skins expressed CK1/CK10 and CK5/CK14.Conclusions Keratinocytes before the 3rd passage have a better ability in the proliferation and differentiation,and so they are more suitable as seed cells for tissue-engineered skin.
