Immunohistochemical study on the expression of calcium binding proteins (calbindin-D28k, calretinin, and parvalbumin) in the cerebellum of the nNOS knock-out(-/-) mice.
- Author:
Jae Chul LEE
1
;
Yoon Hee CHUNG
;
Yu Jin CHO
;
Jandi KIM
;
Nahee KIM
;
Choong Ik CHA
;
Kyeung Min JOO
Author Information
- Publication Type:Original Article
- Keywords: Calbindin-D28k; calretinin; parvalbumin; cerebellum; neuronal nitric oxide synthase (nNOS) knock-out (-/-) mice
- MeSH: Animals; Calcium; Calcium-Binding Protein, Vitamin D-Dependent; Calcium-Binding Proteins; Cerebellar Cortex; Cerebellum; Homeostasis; Immunohistochemistry; Mice; Neurons; Nitric Oxide; Nitric Oxide Synthase
- From:Anatomy & Cell Biology 2010;43(1):64-71
- CountryRepublic of Korea
- Language:English
- Abstract: Nitric Oxide (NO) actively participates in the regulation of neuronal intracellular Ca2+ levels by modulating the activity of various channels and receptors. To test the possibility that modulation of Ca2+ buffer protein expression level by NO participates in this regulatory effect, we examined expression of calbindin-D28k, calretinin, and parvalbumin in the cerebellum of neuronal NO synthase knock-out (nNOS(-/-)) mice using immunohistochemistry. We observed that in the cerebellar cortex of the nNOS(-/-) mice, expression of calbindin-D28k and parvalbumin were significantly increased while expression of calretinin was significantly decreased. These results suggest another mechanism by which NO can participate in the regulation of Ca2+ homeostasis.
