Chromatin organization and transcriptional activation of Hox genes.
- Author:
Ji Yeon LEE
1
;
Hyehyun MIN
;
Xinnan WANG
;
Abdul Aziz KHAN
;
Myoung Hee KIM
Author Information
- Publication Type:Original Article
- Keywords: Hox genes; chromosome conformation capture; spatial organization; chromosomal interaction; gene expression
- MeSH: Axis, Cervical Vertebra; Chromatin; Embryonic Development; Female; Gene Expression; Genes, Homeobox; Pregnancy; Teratocarcinoma; Transcriptional Activation
- From:Anatomy & Cell Biology 2010;43(1):78-85
- CountryRepublic of Korea
- Language:English
- Abstract: Spatially and temporally programmed expression of the Hox genes along the antero-posterior (A-P) axis is essential for correct pattern formation during embryonic development. An accumulating body of evidence indicates the pivotal role of spatial chromatin organization for the coordination of gene regulation. Recently, chromosome conformation capture (3C) technique has been developed and opened a new way to study chromosomal interactions in the nucleus. In this study, we describe 3C method we applied in F9 embryonic teratocarcinoma cells and demonstrate that the chromosomal interactions at Hox loci are successfully detected. Interestingly, at Hoxc loci, the abundance of intrachromosomal interactions with neighboring fragments was drastically decreased when the genes are expressed. These results indicate the possibility of the dynamic pattern of chromosomal interaction in association with the transcriptional regulation of Hox genes.
