An experimental study for prefabrication of skin flap with frozen allogeneic bone

VernacularTitle:异体骨预制骨皮瓣的实验研究
Author: Wei LI ; Qingluan HAN ; Yu ZHANG ; Lei CHEN ; Cunmin RONG ; Jinghua CHEN
Publication Type:Journal Article
Keywords: Allogeneic bone; Prefabrication; Bone-skin flap; Pig
From: Chinese Journal of Microsurgery 2013;(3):257-260
CountryChina
Language:Chinese
Abstract: Objective To research the feasibility of the prefabrication of skin flap with frozen allogeneic bone.Methods From November 2010 to July 2011,fifteen miniature pigs were randomly divided into three groups(n =5) according to different implanted positions.Rewarming,deep-frozen allogenic bone was implanted in subcutaneous tissue,subfascial compartment and muscle respectively.Emission Computed Tomography (ECT) was used in the 4th week,the 8th week and the 12th week postoperatively.Angiography and pathological examination was used in the 12th week.Results The difference of the result of ECT was statistically significant betweent the 4th week and the 8th week (P ＜ 0.05),and there was not statistically significant difference betweent the 12th week and the 8th week (P ＞ 0.05).Angiography showed that all allogeneic bone was vascularized in each group.Pathological examination showed that the vascularization and the inducing osteogenesis of the allogeneic bone which was implanted in the subfascial compartment or muscle was better than that in the subcutaneous tissue.Conclusion The deep-frozen allogenic bone can be used for the prefabrication of the bone-skin flap.