miR-146a regulates monocyte hyper-reactivity to LPS and Poly I : C in patients with primary biliary cirrhosis

VernacularTitle:miR-146a调节原发性胆汁性肝硬化患者单核细胞对LPS以及Poly I∶C的反应
Author: Yuanlan HUANG ; Yan CHEN ; Yi SUN ; Anmei DENG ; Renqian ZHONG
Publication Type:Journal Article
Keywords: miR-146a; Primary biliary cirrhosis; Innate immunity; Inflammatory factors
From:Chinese Journal of Microbiology and Immunology 2013;(5):339-345
CountryChina
Language:Chinese
Abstract: Objective To investigate whether the hyper-reactivity of monocytes from the patients with primary biliary cirrhosis (PBC) to LPS and Poly I ∶ C was associated with miR-146a.Methods Peripheral blood mononuclear cells from PBC patients and healthy controls were stimulated with 10 μg/ml of LPS or Poly I ∶ C.The levels of IL-1 β,IL-6,TNF-α and IFN-α in the culture medium were detected by ELISA.The relative expressions of miR-146a,miR-21,let-7e,miR-155 and miR-125b were detected by RT-PCR.The regulatory role of miR-146a on the production of inflammatory factors in LPS or Poly I ∶ C stimulated THP-1 cells was studied by gain-and-loss function assay.Results The up-regulation of miR146a,which was induced by both LPS or Poly I ∶ C,was impaired in the monocytes from PBC patients.The production of LPS or Poly I ∶ C induced inflammatory factor,could be enhanced by miR-146a down-regulation.Conclusion The hyper-reactivity of monocytes from PBC patients to LPS and Poly I ∶ C was associated with miR-146a.