Relationship analysis of the expressions of heat shock protein 70 and heat shock protein 90 genes in bone marrow mononuclear cells with glucocorticoid-resistence in acute lymphoblastic leukemia patients
10.3760/cma.j.issn.1008-1372.2013.04.015
- VernacularTitle:热休克蛋白70、热休克蛋白90基因在急性淋巴细胞白血病骨髓单个核细胞的表达及其与糖皮质激素抵抗的关系
- Author:
Ying LI
;
Jiheng LIU
;
Jing LIU
- Publication Type:Journal Article
- Keywords:
HSP70 heat-shock proteins/genetics;
HSP90 heat-shock proteins/genetics;
Precursor cell lymphoblastic leukemia-lymphoma;
Glucocorticoids
- From:
Journal of Chinese Physician
2013;(4):486-489
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the relationships of the expressions of heat shock protein 70(HSP70) and HSP90 genes in bone marrow mononuclear cells (BMMNCs) with glucocorticoid (GC)-resistence (GCR) in acute lymphoblastic leukemia (ALL) patients.Methods The expressions of HSP70 and HSP90 in bone marrow mononuclear cells from 43 newly diagnosed ALL patients and 16 healthy volunteers were examined by real time polymerase chain reaction.Of them,43 patients received glucocorticoid therapy were divided into GC-sensitive (GCS) group and GC-resistant(GCR) group according to GC response.Results (1)The expressions of HSP70 mRNA (0.95 ± 0.36) and HSP90 mRNA (1.97 ± 0.63) in BMMNCs from ALL patients were significantly higher than that in the controls [(0.28 ±0.17)and (0.62 ±0.21),P＜0.01]; (2)The expressions of HSP70 mRNA(1.09 ± 0.22)and HSP90 mRNA(2.18 ± 0.42) in BMMNCs from GCR group patients were significantly higher than that in the GCS group patients [(0.57 ±0.19)and (1.43 ± 0.35),P ＜ 0.05,P ＜ 0.01] ; (3)The expressions of HSP70 mRNA (0.97 ± 0.34)and HSP90 mRNA (2.01 ± 0.58) in BMMNCs from the ALL patients with high-degree tumor burden were significantly higher than that in the patients with low-degree tumor burden [(0.61 ±0.22) and (1.34 ±0.29),P ＜0.01].Conclusions The mRNA expressions of HSP70 and HSP90 in BMMNCs from GCR group patients were significantly increased.Detection of HSP90 and HSP70 expressions may help ALL patients for prognosis judgment,chemotherapy plan selection and therapeutic effect evaluation.
