Combined antimicrobial susceptibility test against pan-drug-resistant Acinetobacter baumannii with E-test and microdilution checkerboard assay
10.3760/cma.j.issn.0254-5101.2013.02.015
- VernacularTitle:应用E-test法、肉汤微量稀释棋盘法检测泛耐药鲍曼不动杆菌的试验
- Author:
Tieshan WANG
;
Jianrong SU
- Publication Type:Journal Article
- Keywords:
E-test method;
Broth microdilution checkerboard method;
Time-kill curve;
Pan-drug resistant Acinetobacter baumannii
- From:Chinese Journal of Microbiology and Immunology
2013;(2):144-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the activity of antibiotics against pan-drug-resistant (PDR) Acinetobacter baumannii by combination antimicrobial susceptibility test in viro with epsilometric methods (Etest method) and microdilution checkerboard (CB method),and to detect a good correlation between timekill curve with the above mentioned two assays.Methods Thirty-one clinical isolates of PDR Acinetobacter baumannii were selected for mono and combination antimicrobial susceptibility test in vitro by E-test and CB method,then a comparison was conducted between the test results and the time-kill curve.Mono drugs involved tigecycline,colistin,imipenem and amikacin,and combinations involved two of drugs above,and three drugs involved imipenem/tigecycline,plus amikacin combination.Results Synergistic effect was detected in imipenem plus colistin and tigecycline plus imipenem combination.A high comparability was revealed between the E-test method with antimicrobial drugs added into the culture medium and the time-kill curves.Synergy in the combination of imipenem/tigecycline,plus amikacin was detected by the CB method and time-kill curves.Conclusion The results showed that the effect of specific combination of antibiotics against PDR Acinetobacter baumannii could be predicted by testing their synergistic effect with combination antimicrobial susceptibility test.
