Development and application of one-step polymerase chain reaction(PCR) for rapid identification of Brucella species and some biovars
10.3760/cma.j.issn.0254-5101.2013.02.013
- VernacularTitle:布鲁氏菌种及种内部分生物型快速PCR鉴定方法的建立及应用研究
- Author:
Zhenjun LI
;
Xuexin HOU
;
Weige SUN
;
Xue JIA
;
Guozhong TIAN
- Publication Type:Journal Article
- Keywords:
Brucella;
Polymerase chain reaction;
Identification
- From:
Chinese Journal of Microbiology and Immunology
2013;(2):133-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a one-step PCR assay for rapid discrimination of six Brucella species and some intraspecific biovars.Methods Using 6 pairs of primers in one-step PCR to differentiate six classical Brucella species and some biovar in ordinary PCR instrument.The tested strains including 27 reference strains of six Brucella species and 239 Brucella strains were estimated by the PCR assay and biological identification methods.Results The six Brucella species could be precisely differentiated by the onestep PCR assay from the tested strains.Five biovars and vaccine strain of B.suis species could be determined,and biovars 1,3,4 and biovars 2,5,6,7,9 of B.abortus species could be identified at the level of their biovar,moreover,biovars 1,2 and 3,and vaccine strain Rev 1 of B.melitensis species were also discriminated at the biovar and strain level.The accurate rates of the biological identification method and the PCR assay were 98.33％ and 100％ respectively.Conclusion One-step PCR assay was a rapid,specific,and low cost method for identification of Brucella species and discriminating biovars in ordinary PCR instrument.
