Enhanced killing activity of γδT cells against SW1990 cells by dihydroartemisinin
10.3760/cma.j.issn.0254-5101.2013.02.007
- VernacularTitle:双氢青蒿素对γδT细胞杀伤SW1990细胞的增强作用
- Author:
Bo LU
;
Fuxing CHEN
;
Zhonghai ZHOU
;
Leiqing SUN
;
Sujuan FEI
- Publication Type:Journal Article
- Keywords:
Dihydroartemisinin;
γδT cell;
Cyotoxic activity
- From:
Chinese Journal of Microbiology and Immunology
2013;(2):103-106
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of DHA on proliferation and killing activity of γδT cells against SW1990 cells in vitro.Methods γδT cells were generated in vitro by stimulating peripheral blood mononuclear cells of healthy donors in RPMI 1640 completed medium containing IPP and IL-2 for 8 d,and then co-cultured with different concentrations of DHA for 48 h.Proliferation rates of γδT cells for each group were detected by MTT method.The perforin,granzyme B and CD107a expression in γδT cells were verified by flow cytometer.The cytotoxic activity of γδT cells against SW1990 cells were analyzed by CCK-8 kit.Results The purity of γδT cells in each group reached 75.46％ ±5.32％ after 8 d of culture.Compared with the control group,the proliferative capability of γδT cells were enhanced significantly after treated with 50-100 μmol/ml DHA for 48 h,moreover,cytotoxicity against SW1990 cells and perforin,granzyme B and CD107a expression of the γδT cells treated with DHA were higher than the control group.Conclusion DHA could enhance the antitumor activity of γδT cells,which may be associated with the upregulation of perforin and granzyme B expression in γδT cells.
