Apolipoprotein C3 enhanced THP-1 cell adhesion to aortas of mice

VernacularTitle:载脂蛋白C3促进THP-1细胞向小鼠主动脉黏附
Author: Yaozhong XU ; Juan YU ; Yisong XIONG ; Shaopeng CHU ; Huimin WANG ; Renqian ZHONG
Publication Type:Journal Article
Keywords: Apolipoprotein C3; Adhesion effect; Aorta
From:Chinese Journal of Microbiology and Immunology 2013;(2):99-102
CountryChina
Language:Chinese
Abstract: Objective To investigate the enhancing effect of apolipoprotein C3 (APOC3) on THP-1 cell adhesion to aortas of mice.Methods Microsurgery was performed to separate the aorta of C57BL/6 mice in sterile condition.After stimulated by APOC3 (100 △g/ml) in vitro for 16 h,the aorta was allowed to adhere for 1 h with CFSE labeled THP-1 cells (1 ×106/ml).Then the adhesion effect was observed,and the expressions of vascular adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) were detected by immunohistochemical method.Results Adhesion effect of the mice aorta with THP-1 cells in the APOC3 stimulated group was stronger than the control group.Both the expressions of VCAM-1 and ICAM-1 in aortas were increased by APOC3,but the former was significantly up-regulated than the latter.Conclusion Apolipoprotein C3 could enhance THP-1 cell adhesion to aortas of mice.