Effect of Cordyceps sinensis on renal fibrosis in 5/6 subtotal nephrectomy rats and its possible mechanism
10.3760/cma.j.issn.1001-7097.2013.05.005
- VernacularTitle:冬虫夏草菌粉对5/6肾大部切除大鼠肾脏纤维化的抑制作用及机制
- Author:
Mingming PAN
;
Minghui ZHANG
;
Haifeng NI
;
Junfeng CHEN
;
Min XU
;
Bicheng LIU
- Publication Type:Journal Article
- Keywords:
Cordyceps sinensis;
Fibrosis;
Transforming growth factor beta1;
Signal pathway
- From:
Chinese Journal of Nephrology
2013;(5):347-351
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Cordyceps sinensis on renal fibrosis and its possible mechanism.Methods Thirty Sprague-Dawley rats were randomly divided into three groups:Sham operation group (Sham,n =10),5/6 subtotal uephrectomy group (SNx,n =10),and 5/6subtotal nephrectomy treated with Cordyceps sinensis group (CS,n =10).Body weights were assessed and 24-hour urine excretion was collected before and every four weeks after surgery.Rats were sacrificed at 12 weeks after surgery.Blood samples were taken for biochemical study,and kidney tissues were used for HE and Masson stains to assess histological changes.Immunohistochemical staining was used to detect the expression of transforming growth factor β1 (TGF-β1) and its receptors of type Ⅰ (TβR Ⅰ),type Ⅱ (TβR Ⅱ).Immunofluorescence was used to detect E-cadherin and α-SMA.The relative protein level of TGF-β1,TβR Ⅰ,TβR Ⅱ,p-Smad2/3,Smad7,E-cadherin,α-SMA were examined by Western blotting.Results CS group had higher body weights and lower urinary protein,BUN and Scr level compared with SNx group.Glomerulosclerosis index and tubulointerstitial injury score were significantly reduced in CS group compared with those in SNx group (all P ＜ 0.05).The protein expressions of TGF-β1,TβR Ⅰ,TβR Ⅱ,p-Smad2/3 were decreased in CS group compared with those in SNx group (all P ＜ 0.05).CS treatment up-regulated the expression of E-cadherin,Smad7 and down-regulated the expression of α-SMA compared with that in SNx group (all P ＜ 0.05).Conclusion Cordyceps sinensis has inhibitory effect on renal fibrosis in 5/6 subtotal nephrectomy rat model,which might be related with the suppression of TGF-β 1 signal pathway.
