Effect of cysteine-rich protein 61 on proliferation and cell cycle in human renal tubular epithelial cells
10.3760/cma.j.issn.1001-7097.2013.04.007
- VernacularTitle:富含半胱氨酸蛋白61对人肾小管上皮细胞增殖与细胞周期的影响
- Author:
Yan XU
;
Xuefei SHEN
;
Nianhua SONG
;
Daoxu WU
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Cell cycle;
Focal adhesion protein-tyrosine kinases;
Cyclin-dependent kinases;
Cysteine rich protein 61;
Human renal tubular epithelial cells
- From:
Chinese Journal of Nephrology
2013;(4):273-276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cysteine-rich protein 61 (Cyr61) on proliferation and cell cycle in human renal tubular epithelial cells (HK-2).Methods Cyr61 cDNA was cloned into pEGFP-N2,then HK-2 cells were transfected with the recombinant plasmid pEGFP-N2-Cyr61 by Lipofectamine.The cell proliferation was measured by MTT.The expression level of Cyr61,p-FAK and cyclin dependent cyclin-dependent kinase 2 (CDK2) protein were detected by Western blotting.The cell cycle and cell apoptosis were analyzed by flow cytometry.Results The recombinant plasmid pEGFP-N,-Cyr61 could be transfected into HK-2 efficiently.After transfection,the proliferative activity was significantly increased,the proportion of HK-2 cells in G1 phase decreased and in S-phase increased significantly,the level of cell apoptosis decreased markedly (all P ＜ 0.01).The expressions of Cyr61,p-FAK and CDK2 in Cyr61-transfected group were all amplified significantly (all P ＜ 0.01).Conclusions Cyr61 protein over-expressed in HK-2 cells can increase CDK2 expression throngh FAK pathway,resulting in the promotion of HK-2 cells entering into S phase,cell proliferation and the reduction of cell apoptosis.
