Proteomic analysis of plasma membrane from fluconazole-resistant Candida albicans
10.3760/cma.j.issn.0412-4030.2013.04.011
- VernacularTitle:白念珠菌对氟康唑耐药的差异性膜蛋白质组学分析
- Author:
Xiaoli ZHANG
;
Laiqiang GAO
;
Xifu WU
;
Hong ZHANG
;
Gehua ZHANG
;
Xingfeng YIN
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Fluconazole;
Drug resistance,fungal;
Membrane proteins;
Proteomics
- From:
Chinese Journal of Dermatology
2013;(4):262-265
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and analyze plasma membrane proteins differentially expressed between fluconazole-sensitive and-resistant C.albicans strains.Methods Two C.albicans strains from a same parent,including the fluconazole-sensitive C.albicans strain CA-3 and fluconazole-resistant C.albicans strain CA-16,served as the subject of this study.Plasma membrane proteins were isolated from both of the C.albicans strains,and subjected to two-dimensional polyacrylamide gel electrophoresis analysis for the screening of differentially expressed proteins,which were then identified by using matrix assisted laser desorption/ionization time-of-flight mass spectrometry.The resultant data were searched against a protein database for C.albicans.Results Twentytwo proteins were identified to be differentially expressed between the fiuconazole-resistant and-sensitive C.albicans strain.Of them,6 proteins (Adh1p,Csp37p,Pgk1p,Pgk1p and 2 unnamed proteins,i.e.,gi227305312and gi53954641) were highly expressed,while 16 proteins (Aco1p,Aco1p,Hsp78p,Gut2p,Sdh12p,Ilv2p,Ndh51p,Ndh51p,Atp1p,Pda1p,Srb1p,Idh1p,Tdh1p,Cyt1p,Cox4p,Cox13p) were lowly expressed in the fluconazole-resistant C.albicans strain compared with the fluconazole-sensitive strain.Conclusion The plasma membrane proteins differentially expressed between fluconazole-sensitive and-resistant C.albicans strain are mainly implicated in energy metabolism and mitochondrial function.
