Effects of propofol on airway hyperresponsiveness and airway inflammation of asthmatic mice
10.3760/cma.j.issn.0254-1416.2013.03.020
- VernacularTitle:异丙酚对哮喘小鼠气道高反应性和气道炎症的调节作用
- Author:
Yi ZOU
;
Jing ZHAO
;
Lijiao XING
;
Xin XIN
;
Baosen PANG
;
Kewu HUANG
;
Yuguang HUANG
- Publication Type:Journal Article
- Keywords:
Asthma;
Propofol;
Respiratory hypersensitivity;
Inflammation
- From:
Chinese Journal of Anesthesiology
2013;(3):334-337
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunoregulatory effects of propofol on airway hyperresponsiveness,airway inflammation and Thl/Th2 ratio in the asthmatic mice.Methods One hundred female BALB/c mice,aged 6-8 weeks,weighing 18-20 g,were randomly divided into 5 groups (n =20,each):control group (normal saline i.p.,group C),asthma group (group A),low-dose propofol (50 mg/kg i.p.,group LP),medium-dose propofol (100 mg/kg i.p.,group MP) and high-dose propofol (150 mg/kg i.p.,group HP).Mice of groups A,LP,MP and HP were sensitized with ovalbumin (OVA),mice of group C were sensitized with normal saline.24 h after the last challenge,animals were sacrificed by lethal dose of pentobarbital sodium.Blood and bronchoalveolar lavage fluid (BALF) were collected for determination of serum OVA-specific IgE and the levels of cytokines (IL-4,IL-5 and IFN-γ) in the BALF.Airway responsiveness was measured by the forced-oscillation technique and histological inflammation scores were measured by staining with hematoxylin and eosin.Results Propofol (group LP and group MP) attenuated airway hyperresposiveness to the muscarinic agonist methacholine in OVA-induced asthma.Different doses of propofol (group LP,group MP and group HP) decreased eosinoplils influx in lungs.In addition,propofol treatment reduced expression of IL-4,IL-5 and serum OVA-specific IgE and increased the ratio of IFN-γ/IL-4.Conclusion The study demonstrates a potential protective value of propofol in alleviating airway inflammation,up-regulating Th1/Th2 ratio and attenuating airway hyperresposiveness in the asthmatic mice.
