Effect of dexmedetomidine pretreatment on lipopolysaccharide-induced release of inflammatory mediators in primary microglias in neonatal rats
10.3760/cma.j.issn.0254-1416.2013.03.008
- VernacularTitle:右美托咪定预先给药对脂多糖诱导乳鼠原代小胶质细胞炎性介质释放的影响
- Author:
Mian PENG
;
Yanlin WANG
;
Chengyao WANG
;
Chang CHEN
- Publication Type:Journal Article
- Keywords:
Dexmedetomidine;
Inflammation;
Cytokine;
Microglia
- From:
Chinese Journal of Anesthesiology
2013;(3):296-298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of dexmedetomidine pretreatment on lipopolysaccharide (LPS)-induced release of inflammatory mediators in primary microglias in neonatal rats.Methods Purified primary microglias were seeded in the plate and randomly divided into 3 groups with 10 holes in each group:control group (group C),LPS group (group L) and dexmedetomidine pretreatment group (group D).In group C,the cells were incubated in serum-free DMEM for 24 h.In group LPS,the cells were incubated with LPS (final concentration 1 μg/ml) for 24 h.In group D,the cells were pretreated with dexmedetomidine (final concentration 1 ng/ml) for 1 h,then LPS (final concentration 1 μg/ml) was added and the cells were incubated for 24 h.The levels of nitric oxide (NO) (by Greiss method) and prostaglandin E2 (PGE2),IL-1β and TNF-α (by ELISA) in the supernatant were measured after 24 h of incubation.The expression of inducible nitric oxide synthase (iNOS) mRNA in the cells was determined by RT-PCR.Results Compared with group C,the levels of NO,PGE2,IL-1β and TNF-α,and expression of iNOS mRNA were significantly increased in groups L and D (P ＜ 0.05).There was no significant difference in the indexes mentioned above between groups D and L (P ＞ 0.05).Conclusion Pretreatment with dexmedetomidine has no significant effect on LPS-induced release of inflammatory mediators in primary microglias in neonatal rats.
