Effect of hydrogen-rich saline on lipopolysaccharide-induced acute lung injury in mice
10.3760/cma.j.issn.0254-1416.2013.02.032
- VernacularTitle:富氢液对脂多糖致小鼠急性肺损伤的影响
- Author:
Hongwei LIU
;
Hongguang CHEN
;
Keliang XIE
;
Huanzhi HAN
;
Guolin WANG
;
Yonghao YU
- Publication Type:Journal Article
- Keywords:
Hydrogen;
Respiratory distress syndrome,adult;
Endotoxemia
- From:
Chinese Journal of Anesthesiology
2013;(2):249-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hydrogen-rich saline on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.Methods Thirty-two male C57BL/6 mice,weighing 20-25 g,were randomly divided into 4 groups (n =8 each):control group (group C),hydrogen-rich saline group (group H2),ALI group and ALI + hydrogen-rich saline group (group ALI+ H2).ALI was induced by inhaled aerosolized LPS 25μg (dissolved in PBS) in groups ALI and ALI+ H2.Aerosolized PBS 50μl was inhaled in groups C and H2.0.6mmol/L hydrogen-rich saline 5 ml/kg was injected intraperitoneally at 1 and 12 h after aerosolized PBS or LPS was inhaled in groups H2 and ALI+ H2,respectively.At 24 h after PBS or LPS treatment,the animals were mechanically ventilation for 15 min,and arterial blood gas analysis was performed.The oxygenation index (PaO2/FiO2) was calculated.Bronchoalveolar lavage fluid (BALF) was collected for determination of total protein concentration and polymorphonuclear neutrophils (PMN) count.The concentrations of TNF-α,IL-1β,IL-6 and high mobility group box-1 (HMGB-1) protein in BALF were determined by ELISA.Then the animals were sacrificed and the lungs were obtained for determination of W/D lung weight ratio (W/D ratio),and myeloperoxidase (MPO) and caspase-3 activities.The pathological changes of the lung were scored.Apoptosis index (AI) was calculated.Results Compared with group C,no significant difference was found in PaO2/FiO2,the concentrations of protein,TNF-α,IL-1β,IL-6 and HMGB1,and PMN count in BALF,lung injury score,W/D ratio,MPO and caspase-3 activities and AI in group H2 (P ＞ 0.05),and PaO2/FiO2 was significantly decreased,the concentrations of total protein,TNF-α,IL-1β,IL-6 and HMGB1,and PMN count in BALF,lung injury score,W/D ratio,MPO and caspase-3 activities,and Al were increased in groups ALI and ALI + H2 (P ＜ 0.05).Compared with group ALI,PaO2/FiO2 was signifieantly increased,the concentrations of total protein,TNF-α,IL-1β,IL-6 and HMGB1,and PMN count in BALF,lung injury score,W/D ratio,MPO and caspase-3 activities,and AI were decreased in group ALI+ H2 (P ＜ 0.05).Conclusion Hydrogen-rich saline can alleviate LPS-induced ALI via inhibition of inflammatory response and apoptosis in mice.
