A study on the rapid eradication of tumor cells on the surgical tools used in hepatocellular carcinoma resection
10.3760/cma.j.issn.1007-8118.2013.05.003
- VernacularTitle:原发性肝细胞癌切除术中快速处理肿瘤细胞污染器械的方法研究
- Author:
Xiaofen YU
;
Zaiyuan YE
;
Xianqin HU
;
Yingyu MA
;
Qinfang ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Tumor-free technique;
Operation instrument;
Saline;
Distilled water
- From:
Chinese Journal of Hepatobiliary Surgery
2013;(5):329-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study investigated a method to rapidly inactivate tumor cells on surgical instruments intraoperatively.Methods Tumor cells were collected by immersing and washing the surgical instruments in 37 ℃ saline.The precipitation was collected by low speed centrifugation and then was cultured to harvest the tumor cells.The tumor cells were immersed in saline and distilled water of different temperatures for different duration of time.Inverted microscopy was used to investigate the changes in morphology.Results After immersion in 55 ℃ distilled water for 60 seconds,the tumor cells were swollen,the cell membranes disappeared,the sizes of the nuclei were reduced,the chromatin was condensed,and some cells lysed and separated from each other.Additionally,these tumor cells floated in the culture medium and lacked any living cells adhering to the walls of the bottle.In the group of tumor cells treated with 55 ℃ saline for 60 seconds,there were no obvious morphological changes of the tumor cell or nucleus.Conclusion The intraoperative immersion of surgical instruments in 55 ℃ distilled water for 60 seconds could completely inactivate tumor cells.
