The combination of rAAV-anti EGFR with gemcitabine and radiation in pancreatic cancer
10.3760/cma.j.issn.1007-8118.2013.04.014
- VernacularTitle:抗表皮生长因子受体单链抗体联合放化疗治疗裸鼠移植胰腺癌的研究
- Author:
Tianxiao WANG
;
Jianwei XU
;
Lianfang ZHENG
;
Shiqi WAN
;
Taiping ZHANG
;
Yupei ZHAO
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Epidermal growth factor receptor(EGFR);
Gene therapy;
Radiotherapy;
Drug therapy
- From:
Chinese Journal of Hepatobiliary Surgery
2013;(4):291-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective EGFR targeted therapy mediated by adeno-associated virus is a promising way to treat pancreatic cancer.This study aimed to assess the feasibility and activity of combining rAAV-anti EGFR,gemcitabine,and radiation in pancreatic cancer cells.Methods Aspc-1 human pancreatic carcinoma cells were divided into several groups,in vitro and in vivo,which were respectively exposed to gemcitabine alone,radiation alone,rAAV-anti EGFR alone,the combination of rAAV-anti EGFR with gemcitabine,the combination of rAAV-anti EGFR with radiation,and the combination of all three agents.The pancreatic cancer tumor growth and apoptotic rate were measured.Results The apoptotic rate was higher in cells treated with a single or combination of agents compared to the negative control (P＜0.05).The combination of rAAV-EGFR,gemcitabine,and radiation produced the highest induction of apoptosis compared to a single agent alone (P ＜ 0.05).Treatment with rAAV-anti EGFR greatly inhibited growth in the tumor xenografts (P＜0.05),and a synergistic effect of rAAV-anti EGFR,gemcitabine,and radiation was found.The number of tissue cancer cells that expressed cleaved caspase-3 after treatment with rAAV EGFR was more than that of the control group (P＜0.05).The combined treatment of rAAV-anti EGFR,gemcitabine,and radiation induced the highest numbers of cells expressing cleaved caspase-3 compared to that with a single agent alone (P＜0.05).Conclusions The rAAV-anti EGFR therapy in combination with chemotherapy and radiation therapy demonstrated a greater efficacy over therapy with a single agent alone.rAAV-anti EGFR increased the efficacy of gemcitabine and radiation in the treatment of pancreatic cancer cells.
