Promoter methylation of RASSF1A gene in hepatocellular carcinoma and its clinical significance
10.3760/cma.j.issn.1007-631X.2013.04.016
- VernacularTitle:原发性肝癌RASSF1A基因启动子区甲基化及其临床意义
- Author:
Tangen CHEN
;
Jianguo LI
;
Zhichuan LIN
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
DNA methylation;
RASSF1A
- From:
Chinese Journal of General Surgery
2013;(4):300-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between promoter methylation of RASSF1 A gene and clinico-pathological characteristics in hepatocellular carcinoma.Methods MS-PCR was used for analyzing the status of aberrant promoter methylation of RASSF1A in 100 primary HCC samples and adjacent noncancerous tissues,10 normal liver tissues,and six HCC cell lines.RT-PCR was used to assess reactivation of RASSF1A expression after HCC cell lines treated with demethylating agent 5'-aza-2' deoxycytideing.Results Abnormal promoter methylation of RASSF1A gene was found in 69(69%) cases of HCC,15 (15%) cases of adjacent normal tissues,and no abnormal promoter methylation of RASSF1A gene was found in normal liver tissues,and the difference was statistically significant (x2 =67.75,P <0.001).The methylation of RASSF1A gene was correlated to HBsAg (x2 =11.341,P < 0.05) and histological differentiation(x2 =10.575,P < 0.05).Four cell lines with abnormal CpG island methylation of RASSF1A gene were all re-expressed after treated with 5'-Aza-CdR.Conclusions RASSF1A gene promoter methylation was correlated to HBsAg and histological differentiation and this is one of the most important mechanism for low expression of RASSF1A in HCC.