T cell and CD4+ CD28-T cell changes in the occurrence of rat abdominal aortic aneurysm
10.3760/cma.j.issn.1007-631X.2013.04.015
- VernacularTitle:大鼠腹主动脉瘤形成过程中T细胞及CD4+CD28-T细胞的动态变化及意义
- Author:
Wenping XU
;
Zhiquan XIE
;
Zhiliang LI
;
Jian QIU
;
Ziqiang WU
;
Ruibin FU
- Publication Type:Journal Article
- Keywords:
Aortic aneurysm,abdominal;
T-lymphocytes;
Inflammation;
Immunity
- From:
Chinese Journal of General Surgery
2013;(4):296-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate T cell and CD4+ CD28-T in the development and progression of abdominal aortic aneurysms (AAAs).Methods Fifty Wistar rats were randomly divided into 5 groups (n =10 each).An AAA animal model is established by enhancing perfusing elastase to the infrarenal abdominal aorta of the rats.The levels of T cell,B cell and macrophage cell of abdominal aorta of the rats on days 3,7,14 and 28,were detected by enzyme linked immunosorbent assay(ELISA).CD4+ CD28-T cell of the peripheral blood were measured by flow cytometry.Result There was significant T-lymphocyte infiltration both in middle and outer membrane of the artery of the rats on day 7 after surgery.T-lymphocyte,B-lymphocyte and macrophage cell infiltration were on the peak in middle and outer membrane of the artery on day 14 after surgery.The ratio of CD4+ CD28-T cell in rat peripheral blood reached peak on day 7(P ＜0.05).Conclusions T cell and CD4+ CD28-T cell expression increased in peripheral blood and abdominal aorta in AAA rat model,suggesting a potential role of T cell and CD4+ CD28-T cells in the pathogenesis of AAAs,especially during the early development of AAAs.
