Diagnostic value of immediate early antigen-mRNA in the diagnosis of cytomegalovirus infection after kidney transplantation
10.3760/cma.j.issn.1008-6315.2013.07.015
- VernacularTitle:即刻早期抗原-mRNA测定对肾移植后巨细胞病毒感染的诊断价值
- Author:
Chuan ZHANG
;
Yong SHAO
;
Zhizhong GAO
;
Minggang ZHANG
;
Wentao WANG
;
Yunli LIU
- Publication Type:Journal Article
- Keywords:
Kidney transplantion;
Cytomegalovirus infection;
Nucleic acid sequence-based amplification;
Real time-PCR;
Enzyme-linked Immunosorbent assay
- From:
Clinical Medicine of China
2013;(7):714-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic value of the immediate early antigen(IE) mRNA by nucleic acid sequence-based amplification(NASBA) in peripheral blood cytomegalovirus (CMV) infection,and to establish and promote the diagnosis method for CMV.Methods Five to seven ml blood was taken from 32 patients at 3 week and 7 week after renal transplantation to detect serum cytomegalovirus antigen and antibody expression by NASBA,Real time-PCR and enzyme-linked immunosorbent assay (ELISA) sensitivity and specificity were compared.Results The results of CMV detection in 32 renal transplanted patients respectively showed that the positive rate of peripheral blood IE-mRNA by NASBA was 45.8％ (15/32) ;The positive rate of HCMV-DNA in blood by Real time-PCR was 45.8％ (15/32).Using ELISA,the positive rate of HCMV-(IgG +IgM) was 37.5％ (14/32).IE-mRNA and HCMV-DNA had higher sensitivity and specificity and lower false positive rate than HCMV-(IgG +IgM).The positive rates of IE-mRNA by NASBA,Real time-PCR and ELISA were 92.8％,71.5％ and 42.8％ respectively in the 14 cases.Conclusion The nucleic acid amplification method (NASBA based sequence) and Real time-PCR are sensitive,rapid diagnosis methods of HCMV infection,with higher sensitivity and specificity and lower false positive rate than traditional ELISA.And NASBA detection of IE-mRNA has good value for auxiliary clinical diagnosis.
