Preliminary analyses of lymphocyte differentially expressed proteins in Huaihua Dong peoples with hypertension
10.3760/cma.j.issn.1008-1372.2013.05.006
- VernacularTitle:怀化侗族高血压人群血淋巴细胞差异蛋白质表达谱的分析
- Author:
Ling JIN
;
Weiwu LIU
;
Zi XING
;
Yufeng DUAN
;
Hongliang LI
- Publication Type:Journal Article
- Keywords:
Hypertension;
Proteomics;
Mass spectrometry;
Minority groups
- From:
Journal of Chinese Physician
2013;(5):599-603
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore lymphocyte differentially expressed protein profile and its pathogenic significance in Huaihua Dong peoples with hypertension.Methods The lymphocyte protein profiles among 130 cases of blood specimen were detected and identified with matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF-MS) and Mascot search against protein database.Results The reproducible protein-maps of two-dimensional gel electrophoresis were obtained among three groups.Compared with Dong normal peoples,the protein disulfide isomerase-related protein 5 and heat shock protein 27 showed higher expressions in the Dong peoples with hypertension (P ＜ 0.01),but chain A,structure of haemoglobin in the deoxy quaternary state with ligand bound at the alpha haems is no statistical significance(P ＞0.05).Those three proteins were upregulated in Han peoples with hypertension (P ＜0.01).Conclusions The up-regulation of the protein disulfide isomerase-related protein 5 and heat shock protein 27 in the Dong and Han peoples with hypertension suggest that those two proteins might be associated with the occurrence and development of hypertension.
