Analysis of human papillomavirus 16 subtypes E2 gene and long control region sequence in Chengdu

VernacularTitle:成都地区人乳头瘤病毒16型E2基因和长控制区序列分析
Author: Gangyi CHEN ; Xianping DING ; Lin CHEN ; Qiang QUAN ; Shuangshuang NIE
Publication Type:Journal Article
Keywords: Genes,viral; Binding sites; Human papillomavirus 16; Mutation; Sequence analysis
From: Chinese Journal of Infectious Diseases 2013;(5):290-294
CountryChina
Language:Chinese
Abstract: Objective To explore the relevance between sequence variation of human papillomavirus (HPV)16 subtypes E2 gene or long control region (LCR) and cervical lesions.Methods Fifty specimens from HPV16 infected people in Chengdu were collected,including cervical exfoliated cells from 38 HPV carriers,papilloma tissues from 8 cases of genital warts,2 with cervical intraepithelial neoplasia (CIN) Ⅱ and 2 with CIN Ⅲ in this study.Polymerase chain reaction was used to amplify E2 gene and LCR,then an evolutionary tree was constructed.Results In all the 50 specimens,there were 12 mutation sites in E2 gene,among which,C→A existed in one specimen of genital warts,and ≥2 mutation sites existed in all the other 48 specimens.There were 28 mutation sites of LCR sequence of all the specimens.Ten specimens were chosen to construct evolutionary tree and were sequenced.The data showed that 8 specimens were Asian variants,E2 gene mutation existed in all the specimens while the LCR 7867 G→A only existed in the four CIN.Conclusion LCR 7867G→A is a correlative mutation site of cervical lesions in Chengdu.