Effect of Smo siRNA on the aggressive capability of human esophageal carcinoma EC9706 cells
10.3760/cma.j.issn.1008-1372.2013.06.005
- VernacularTitle:Smo siRNA对人食管癌EC9706细胞侵袭力的影响
- Author:
Ling SONG
;
Qiang ZHOU
;
Kuisheng CHEN
;
Zhihua ZHAO
- Publication Type:Journal Article
- Keywords:
RNA,small Interfering;
Esophageal neoplasms/pathology;
Esophageal neoplasms/genetics;
Neoplasm invasiveness
- From:
Journal of Chinese Physician
2013;(6):736-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Smo siRNA on the aggressive capability of human esophageal carcinoma EC9706 cells.Methods EC9706 cells were transfected by Smo siRNA.The expressions of Smo and Glil protein in experimental and control groups were detected with immunocytochemistry.The expressions of Smo and Gli1 mRNA in experimental and control groups were detected with in situ hybridization.The change of aggressive capability in all groups was detected by Boyden chamber in vitro.Results Compared with control group,there was a significant decrease in the protein and mRNA levels of Smo and Gli1 in every specific siRNA transfection group with three different concentration (P < 0.05).The aggressive capability of EC9706 cells was significantly weakened after the transfection (P < 0.05).Conclusions Smo siRNA can inhibit the expressions of Smo and Gli1 genes in EC9706 cells,and can also weaken the aggressive capability of EC9706 cells.